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The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response.Cell Res., March, 2016.
2016-03-11 | A A A  [print][close]
 

By Yang Y1, Wang SY1, Huang ZF1, Zou HM1, Yan BR2, Luo WW2, Wang YY1.

 

Abstract

 

Recognition of viral dsRNA by Toll-like receptor 3 (TLR3) leads to induction of interferons (IFNs) and proinflammatory cytokines, and innate antiviral response. Here we identified the RNA-binding protein Mex3B as a positive regulator of TLR3-mediated signaling by expression cloning screens. Cells from Mex3b(-/-) mice exhibited reduced production of IFN-β in response to the dsRNA analog poly(I:C) but not infection with RNA viruses. Mex3b(-/-) mice injected with poly(I:C) was more resistant to poly(I:C)-induced death. Mex3B was associated with TLR3 in the endosomes. It bound to dsRNA and increased the dsRNA-binding activity of TLR3. Mex3B also promoted the proteolytic processing of TLR3, which is critical for its activation. Mutants of Mex3B lacking its RNA-binding activity inhibited TLR3-mediated IFN-β induction. These findings suggest that Mex3B acts as a coreceptor of TLR3 in innate antiviral response.

 

·         1Wuhan Institute of Virology, State Key Laboratory of Virology, Chinese Academy of Sciences, Hubei 430071, China.

·         2Collaborative Innovation Center for Viral Immunology, Medical Research Institute, College of Life Sciences, Wuhan University, Hubei 430072, China.

 

The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response.

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