Zika virus (ZIKV) is an emerging arthropod-born virus (arbovirus) belonging to the genus Flavivirus of the family Flaviviridae, which also includes a group of human pathogenic viruses such as dengue virus 1-4 (DENV 1-4), West Nile virus (WNV), Japanese encephalitis virus (JEV), yellow fever virus (YFV), and tick-borne encephalitis virus (TBEV). In early 2015, a ZIKV infection outbreak was recognized in northeast Brazil, which posed great threats to the human health. Many laboratories have screened the host factors that ZIKV replications rely on. However, questions regarding how host proteins are regulated during ZIKV infection at protein level remain unknown.

Mosquitoes are epidemiologically important vectors for ZIKV, and effective restrictions of ZIKV replication in mosquitoes will be effective in controlling the spread of the virus. To identify proteins and pathways involved in ZIKV life cycles in mosquito cells, a quantitative proteomic analysis of ZIKV infected C6/36 cells was performed by the research group led by Prof. XIAO Gengfu from Wuhan Institute of Virology of the Chinese Academy of Sciences. The scientists quantified 3,544 host proteins, with 200 being differentially regulated. Bioinformatics analysis revealed that several ZIKV regulated biological processes. In addition, the further research indicated ubiquitin proteasome system (UPS) play roles in ZIKV entry process, and an FDA approved inhibitor of the UPS, Bortezomib, can inhibit ZIKV infection in vivo.

This is the first reported quantitative proteomic analysis of ZIKV infected host cells. To better understand the mosquito cell response to ZIKV infection, a map was created. In addition, this study provides a candidate drug for the control of ZIKV infection in mosquitoes and treatment of ZIKV infection in patient.

The results have been published in Journal of Virology entitled "Quantitative proteomic analysis of mosquito C6/36 cells reveals host proteins involved in Zika virus infection ". The Editor Michael Diamond, a famous virologist evaluated,“This could be an important paper for the journal.”

This work was supported by Ministry of Science and Technology of China, Chinese Academy of Sciences and the National Natural Science Foundation of China.

Global view of host proteins regulated in ZIKV infected C6/36 cells.  Image by XIAO Gengfu

Contact:
XIAO Gengfu
E-mail: xiaogf@wh.iov.cn
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. （http://english.whiov.cas.cn/）