Title: Principal Investigator E-mail: qfang@wh.iov.cn
Education
Chen CL, Sun XY, Liao LJ, Luo SX, Li ZQ, Zhang XH, Wang YP, Guo QL, Fang Q*, Dai HP*. 2013. Antigenic analysis of grass carp reovirus using single chain fragment variable antibody against IgM from Ctenopharyngodon idella. Science China-Life Sciences, 16( 1 ).59-65.
Shao L, Guo H, Yan LM, Liu H, Fang Q*. 2013. Aquareovirus NS80 recruits viral proteins to its inclusions, and its C-terminal domain is the primary driving force for viral inclusion formation. PLoS One, 8(2):e55334.
Guo H, Sun XY , Yan LM , Shao L, Fang Q*. 2012. The NS16 protein of aquareovirus-C is a fusion-associated small transmembrane (FAST) protein, and its activity can be enhanced by the nonstructural protein NS26. Virus Res, doi: 10.1016.
Yan LM, Guo H, Sun XY, Shao L, and Fang Q*. 2012. Characterization of grass carp reovirus minor core protein VP4. Virol J, 9:89.
Shao L., Sun X.Y., Fang Q.*. 2011. Antibodies against outer-capsid proteins of grass carp reovirus expressed in E. coliare capable of neutralizing viral infectivity. Virol J, 8: 347.
Cai L., Sun X.Y., Shao L., Fang Q.*. 2011. Functional investigation of grass carp reovirus nonstructural proteinNS80. Virol J, 8: 168
Fan C., Shao L. and Fang Q.*. 2010. Characterization of the nonstructural protein NS80 of grass carp reovirus. Arch Virol, 155: 1755-1763.
Zhang L.L., Luo Q., Fang Q.* and Wang Y.P.. 2010. An improved RT-PCR assay for rapid and sensitive detection of grass carp reovirus. J Virol Methods, 169: 28-33.
Zhang X., Jin L., Fang Q., Hui W. H. and Zhou Z. H.*. 2010. 3.3 Cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry.Cell, 141: 472-482.
Zhu, J., Cheng, L.P., Fang, Q., Honig, B.* and Zhou, Z.H.*. 2010. Building and refining protein models within cryo-electron microscopy density maps based on homology modeling and multi-scale structure refinement. J Mol Biol, 397: 835-851.
Cheng, L.P., Zhu, J., Hui, W.H., Zhang, X.K., Honig, B., Fang, Q.*and Zhou, Z.H.*. 2010. Backbone model of an aquareovirus virion by cryo-electron microscopy and bioinformatics.J Mol Biol, 397: 852-863.
Shao L., Fan C., Maj E.and Qin Fang*. 2010. Molecular characterization of nonstructural protein NS38 of grass carp reoviru. Virol Sin, 25(2): 123-129.
Fan C., and Fang Q.*. 2009. Functional analyses of mammalian reovirus nonstructural protein μNS. Virol Sin, 24(1): 1-8.
Fan C., Zhang L.L., Lei C.F. and Fang Q.*. 2009. Expression and identification of inclusion forming-related domain of NS80 nonstructural protein of grass carp reovirus. Virol Sin, 24(3): 194-201.
Zhang L.L., Shen J.Y., Lei C.F., Fan C., Hao G.J. and Fang Q.*. 2009. Expression of outer capsid protein VP5 of grass varp reovirus in E.coliand analysis of its immunogenicity. Virol Sin, 24(6): 545-551.
Cheng L.P., Fang Q.*, Shah S., Atanasov C.I. and Zhou Z.H.*. 2008 Subnanometer-resolution Structures of the grass carp reovirus core and virion. J Mol Biol, 382: 213-222.
Zhang L.L., Shen J.Y., Lei C.F., Li X.M. and Fang Q.*. 2008. High level expression of grass carp reovirus VP7 Pprotein in prokaryotic cells. Virol Sin, 23(1): 51-56.
Fang Q*., Seng E.K., Ding Q.Q., Zhang,L.L.. 2008. Characterization of infectious particles of grass carp reovirus by treatment with proteases. Arch Virol, 2008, 153: 675-682.
Jaafar F.M., Goodwin A.E., Belhouchet M., Merry G., Fang Q., Cantaloube J.F., Biagini P., Micco P., Mertens P.P.C. and Attoui H.*. 2008. Complete characterisation of the American grass carp reovirus genome (genus Aquareovirus: family Reoviridae) reveals an evolutionary link between aquareoviruses and coltiviruses. Virology, 373:310-321.
Fang Q*, Seng E.K., Dai W. and Zhang L.L.. 2007. Construction and co-expression of grass carp reovirus VP6 protein and enhanced green fluorescence protein in the insect cells. Virol Sin, 22(5):397-404.
Seng, E.K., Fang Q., Sin Y.M.and Lam T.J*.2005. Molecular cloning, DNA sequence analysis, and expression of cDNA sequence of RNA genomic segment 6 (S6) that encodes a viral outer capsid protein of Threadfin aquareovirus (TFV), Virus Gene, 30(2): 209-221.
Seng E.K, Fang Q., Sin Y.M.,and Lam T.J.2005. Molecular characterization of a major outer capsid protein encoded by the Threadfin aquareovirus (TFV) gene segment 10 (S10) . Arch Virol, 150: 2021–2036.
Seng E.K., Fang Q., Sin Y.M. and Lam T.J. 2004. Development of a rapid, sensitive andspecific diagnostic assay for fish Aquareovirusbased onRT-PCR. J Virol Methods, 118: 111–122.
Seng, E.K., Fang Q., Chang S.F., Ngoh G.H., Qin Q.W., Lam T.J. and Sin Y.M.*. 2002. Characterisation of a pathogenic virus isolated from marine threadfin fish (Eleutheronema tetradactylus)during a disease outbreak. Aquaculture, 214:1-18.
Attoui H.*, Fang Q., Mohd Jaafar F., Cantaloube J.F., Biagini P., Micco P.de and Lamballerie X.de. 2002. Common evolutionary origin of aquareoviruses and orthoreoviruses revealed by genome characterization of golden shiner reovirus, grass carp reovirus, striped bass reovirus and golden ide reovirus (genus Aquareovirus, familyReoviridae). J Gen Virol , 83:1941-1951.
Fang, Q., Xiao T.Y., Ding Q.Q. and Zhu Z.Y.. 2002. Virological properties of GCRV991. Virol Sin, 17:178-181.
Fang, Q., Xiao T.Y., Li L., Zou G.P., Zhang H.Y. and Wang Y.P.. 2002. Infectious characterizations of four grass carp reovirus (GCRV) stains. Virol Sin, 17:182-184.
Fang, Q., Zhu Z.Y.. 2002. Expression of grass carp reovirus (GCRV) RNA dependent RNA polymerase(RdRP) gene in E.coli. Chin J Virol,18(1): 86-88.
Fang, Q., Ai T.S., Wang Y.P., Zhu Z.Y.. 2001. Morphology and morphogenesis of manderin fish virus . Virol Sin, 16:382-385.
Fang, Q.*, Attoui H., Biagini J.F.P., Zhu Z.Y.*, de Micco P. , de Lamba X.. 2000. Sequence of genome segments 1, 2 and 3 of the grass carp reovirus (genus Aquareovirus, family Reoviridae). Biochem Biophys Res Commun, 274: 762-766.
Fang, Q., Ai T.H., Wang Y.P.. 2000. Study of siniperca chuats viral pathogen and its infectious characteristics in fish cells. Virol Sin, 15:297-301.
Zou, G.P., Fang Q.*. 2000. Study on replication and morphogenesis of the grass carp reovirus (GCRV) in CIK cells. Virol Sin, 15: 188-192.
Fang, Q., Tian J.. 2000. Construction of cDNA libraries of GCRV partial genome segments. Virol Sin, 15: 78-82.
Tian, J., Fang Q.. 2000. A rapid, simple and very efficient method for synthesis and cloning of cDNA. Chin Biotechnol, 10: 7-10.
Fang, Q., Jing T.. 1999. Study on cycle sequencing of double stranded DNA template. Chin Biotechnol, 9: 9-12.
Fang, Q., Tian J.. 1999. Improving transformation efficiency of blunting-end ligated vector DNA by electro-transformation. Chin Biotechnol, 9: 5-8.
Tian J., Zou G.P., Fang Q.*. 1999. cDNA synthesis and cloning and partial nucleotide sequence of grass carp hemorrhage virus (GCHV). Virol Sin, 14: 87-92.
Fang, Q., Jiang H., Ke L.H.. 1997. Preparation of grass carp hemorrhage virus antigen subunits. Virol Sin, 11: 77-81.
Wang, W., Zhao T.Y., Fang Q. Jiang H. and Ke L.H.. 1995. Immunogenicity of grass carp hemorrhage virus polypeptides. Virol Sin, 10: 166-168.
Wang, W., Cai Y.Q., Fang Q., Liu Y.W. and Ke L.H.. 1994. Gene-protein-coding assignment of grass carp hemorrhage virus genome RNA species. Virol Sin, 9: 356-361.
Fang, Q., Wu Y.T., Cai Y.Q. 1994. Studies on two-step PCR amplification and rapid detection of HBV-DNA. Virol Sin, 9: 261-265.
Ke, L.H., Yu L.F., Fang Q.,Ding Q.Q. and Cai Y.Q.. 1990. Characteristics of a novel isolate of grass carp hemorrhage virus(GCHV). Acta Hydrabiol Sin, 14: 153-159.
Fang, Q., L. H. Ke, Y. Q. Cai. Growth characteristic and high titer culture of grass carp hemorrhage virus(GCHV). Virol Sin,1989, 4:315-320.